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Figure 2. Transcriptomic profiling reveals extensive but heterogeneous gene expression remodeling in chemoresistant HR+/HER2- BC cells. (A and B) Volcano plots depicting differentially expressed genes in chemoresistant MCF-7 cells (A) and ZR-75-1 (B) cells compared with their respective parental counterparts. Differentially expressed genes were identified using a threshold of FDR < 0.05 without imposing an absolute log₂FC cutoff, and were classified as upregulated (log₂FC > 0) or downregulated (log₂FC < 0) according to the direction of change; (C) UpSet plots illustrating the overlap of differentially expressed genes (logFC < -0.5 or > 0.5; FDR < 0.05) across all chemoresistant models; (D) Heatmap showing genes consistently dysregulated (logFC < -0.5 or > 0.5; FDR < 0.05) across taxane- and anthracycline-resistant HR+/HER2- BC cell models. HR+/HER2-: Hormone receptor-positive/human epidermal growth factor receptor 2-negative; BC: breast cancer; FDR: false discovery rate; Doc-R: docetaxel-resistant; Epi-R: epirubicin-resistant; Pac-R: paclitaxel-resistant.