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Figure 3. LINC01607 boosts migration and invasion of HCC cells. (A) Representative images of HCC cell migration and invasion assessed by Transwell assays after LINC01607 modulation. The quantification of migrating and invading cells is shown; (B) Wound-healing assays showed increased wound closure after LINC01607 overexpression and reduced wound closure after LINC01607 knockdown. Quantification of wound closure is shown; (C) Tail-vein injection models were used to assess lung metastasis in nude mice. Endpoint bioluminescence imaging, gross lung images, metastatic nodule counts, and H&E staining showed greater metastatic burden in the LINC01607-overexpression group. Data are presented as mean ± SD. For migration, invasion, and wound-healing assays, n = 3 independent biological replicates unless otherwise indicated. For the lung metastasis model, n = 6 mice per group. Statistical significance was assessed using two-tailed Student’s t-test for two-group comparisons and one-way ANOVA for multiple-group comparisons, as appropriate. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. HCC: Hepatocellular carcinoma; H&E: hematoxylin and eosin; SD: standard deviation; ANOVA: analysis of variance.