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Figure 2. Hsa-circ-0001030 suppresses proliferation, migration, and cisplatin resistance in TSCC cells. (A and B) Expression of hsa-circ-0001030 and the parental EXOC6B mRNA in Cal27 cells (A) and Tca8113 (B) cells quantified by qRT-PCR analysis following lentiviral overexpression; (C and D) Cell proliferation of Cal27 cells (C) and Tca8113 cells (D) transfected with vector or hsa-circ-0001030 lentivirus, determined by CCK-8 assay; (E and F) Colony formation assays showing reduced clonogenic growth in Cal27 (E) and Tca8113 (F) cells overexpressing hsa-circ-0001030; (G-J) Representative images and quantification of Transwell assays demonstrating that hsa-circ-0001030 overexpression suppresses migration (G, I) and invasion (H, J) of Cal27 and Tca8113 cells; (K) qRT-PCR analysis showing the relative expression levels of hsa-circ-0001030 in parental (Cal27P, Tca8113P) and cisplatin-resistant (Cal27R, Tca8113R) cell lines; (L and M) Measurement of cisplatin IC₅₀ values in Cal27P, Cal27R, Tca8113P, and Tca8113R cells using an MTT assay; (N and O) Representative Transwell images showing enhanced migratory and invasive abilities of cisplatin-resistant Cal27R (N) and Tca8113R (O) cells compared with their parental counterparts. Data are presented as mean ± SD from at least three independent experiments. P < 0.05 was considered statistically significant. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ns: not significant. TSCC: Tongue squamous cell carcinoma; mRNA: messenger RNA; qRT-PCR: quantitative real-time polymerase chain reaction; CCK-8: cell counting kit-8; IC₅₀: half-maximal inhibitory concentration; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; SD: standard deviation.