fig5

Figure 5. Combination of M1 virus and IRE1α inhibitor STF significantly inhibits bladder tumor growth in vivo. Schematic of treatment regimen. STF-083010 (30 mg/kg) was administered intraperitoneally on days 5 and 12. M1 virus (5 × 10⁵ pfu/day) was delivered intravenously for three consecutive days starting on days 6 and 13; (B) Tumor volume growth curves over 21 days; (C and D) Representative images of tumor-bearing mice and harvested tumors on day 21; (E) Final tumor volume measurements; (F) Mouse body weight monitoring across all treatment groups; (G) Immunohistochemical staining of tumor sections for Ki-67 and cleaved caspase-3. Scale bars = 100 μm; (H) Quantification of Ki-67 and cleaved caspase-3–positive cells. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001; N.S.: not significant. IRE1α: Inositol-requiring enzyme 1 alpha.