fig6

Figure 6. Changes in SCFA and BCFA production during in vitro fecal fermentation of chRG-I using fecal samples collected from 15 participants of the intervention study. Fecal inocula were incubated anaerobically with or without chRG-I (blank control). Concentrations of acetate (A), propionate (B), butyrate (C), and total BCFA (D) were determined at 24 h and 48 h and are presented as boxplots showing median, interquartile range, minimum-maximum values and outliers donor values. Differences between chRG-I and blank control were analyzed using paired two-tailed t-tests. *: statistically significant differences compared with the corresponding blank control (P < 0.05). chRG-I: RG-I derived from chicory root; SCFA: short-chain fatty acids; BCFA: branched-chain fatty acids.