fig5

Figure 5. Intracellular levels of photosensitizers in MCF-7 TX400 (P-gp overexpressing) cell line using extraction method. Intracellular levels of the photosensitizers from cell lysates were determined by the fluorescence signals of the photosensitizer. BPD and rose bengal show a significant increase in accumulation in MCF-7 TX400 cells in the presence of a P-gp inhibitor (valspodar). The fmole/mg protein of the photosensitizer was plotted for all the experimental groups. (A) Benzoporhyrin derivative; (B) Temoporfin; (C) Redaporfin; (D) Talaporfin sodium; (E) Methylene blue; (F) Indocyanine green; (G) Rose bengal. *P < 0.05, **P < 0.01, ***P < 0.001; One-way ANOVA Tukey’s range test (N = 3-5). NT: Not treated, control; PS: photosensitizer only; PS+Valspodar: photosensitizer + inhibitor; ns: non-significant; BPD: benzoporphyrin derivative.