fig4

Figure 4. Intracellular levels of photosensitizers in MCF-7 MX100 (ABCG2-overexpressing) cell line using extraction method. Intracellular levels of the photosensitizers from cell lysates were determined by the fluorescence signals of the photosensitizer. BPD and rose bengal show a significant increase in accumulation in MCF-7 MX100 cells in the presence of ABCG2 inhibitor (FTC). The fmole/mg protein of photosensitizer is plotted for all the experimental groups. (A) Benzoporphyrin derivative; (B) Temoporfin; (C) Redaporfin; (D) Talaporfin sodium; (E) Rose Bengal; (F) Methylene blue; (G) Indocyanine green. (*P < 0.05, **P < 0.01, ***P < 0.001; One-way ANOVA Tukey’s range test) (N = 3-6). NT: not treated control; PS: photosensitizer only; PS+FTC: photosensitizer + inhibitor; ns: non-significant; BPD: benzoporphyrin derivative; FTC: fumitremorgin C.