fig3

Figure 3. Normalized absorbance (blue) and fluorescence (red) spectra of (A) BPD, (B) temoporfin, (C) redaporfin, (D) talaporfin sodium, (E) rose bengal, (F) methylene blue and (G) ICG used in the study. The absorbance spectrum of the test photosensitizers was recorded using a spectrophotometer. The photosensitizers were excited at respective Soret and Q bands to record the emission spectrum of the photosensitizers. BPD Ex: 435 nm; Temoporfin Ex: 422 nm; Redaporfin Ex: 510 nm; Talaporfin sodium Ex: 398 nm; Rose bengal Ex: 520 nm; Methylene blue Ex: 610 nm; ICG Ex: 730 nm. Respective excitation wavelengths at the Soret or Q band were chosen to avoid overlap between the excitation and the emission wavelengths while recording the fluorescence intensity during extraction experiments. BPD: Benzoporphyrin derivative; ICG: indocyanine green.