fig1
Figure 1. Schematic of the intracellular photosensitizer accumulation quantification (Extraction) method. 3.0 × 105 MCF-7 MX 100, MCF-7 TX400, and MCF-7/VP cells were plated in 35-mm dishes in duplicate and incubated overnight. After 24 h, the cells were incubated with the desired photosensitizer with or without ABC transporter inhibitors (ABCG2: FTC, P-gp: valspodar or MRP1: MK571) for 1 h at 37 °C in 5% CO2. Cells were washed with PBS and then incubated for 1 h at 37 °C in a photosensitizer-free medium with or without ABC transporter inhibitors to allow efflux. The cells were subsequently washed and collected for cell lysate preparation. The cell lysates were analyzed using BCA assay for protein quantification. The fluorescence intensity of the experimental groups was recorded using the spectrophotometer at specific Ex/Em wavelengths. Created with BioRender.com. FTC: Fumitremorgin C; PBS: phosphate-buffered saline; ABC: ATP binding cassette; BCA: bicinchoninic acid.
