fig1

Figure 1. Representative CTCs isolated in soft-tissue sarcoma patients. CTCs were isolated by using a two-step process, including pre-enrichment by Parsortix^TM, followed by visualisation and isolation with the DEPARarry^TM device after immunostaining. Three CTC populations were isolated: Vim⁺ CK⁺, Vim^- CK⁺, and Vim⁺ CK^-. CTCs: Circulating tumour cells; Vim: vimentin; CK: cytokeratin; DAPI: 4',6-diamidino-2-phehylindole.