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Figure 8. Overexpression of ATF5 alleviates MASLD in mice. (A) ATF5 overexpressing mice at sacrifice; (B) ATF5 overexpression of green fluorescence images; (C) Western blotting analysis of ATF5 overexpression efficiency in mouse liver; (D) Oil red staining of frozen liver sections from high-fat-diet-fed mice and ATF5 overexpressing mice; (E) four groups of mice ITT experiment; (F) Four groups of mice GTT experiment; G-J: Western blot bands and quantitative analysis reveal the expression levels of ATF5, HSP60, and LONP1 proteins in the livers of the four groups of mice. (****P < 0.0001, ***P < 0.001, **P < 0.01, and*P < 0.05.). NCD: Normal chow diet; HFD: high-fat diet; LA: L-arabinose; ATF5: transcription factor 5; HSP60: heat shock protein 60; LONP1: Lon protease 1; AUC: area under curve; ITT: insulin tolerance test; GTT: glucose tolerance test; MASLD: metabolic dysfunction-associated steatotic liver disease.