fig1

Figure 1. Metabolic and gene expression changes induced by WD compared to a ND and WD+Ch compared to only WD in mice. Mice were separated into three experimental groups across two experiments (n = 5 per group, per experiment). Statistics were performed on the pooled samples from both experiments, but the fold change direction for each parameter was required to be consistent across experiments. Data for each experiment are shown separately per treatment group. (A) Summary table of significantly changed host parameters (phenotypes: one-tailed Mann-Whitney U P-value < 0.05) and genes (two-tailed Mann-Whitney U P-value < 0.05) that are consistent in fold change direction across both experiments; (B) GTT plots demonstrating the changes in blood glucose levels over the first 2 h after glucose injection; (C) dot plots of phenotypes and gene expression that demonstrated significant differences in fold changes. Circles represent experiment 1 and triangles represent experiment 2. *P < 0.05; **P < 0.01; ***P < 0.001; †P < 0.1. WD: Western diet; ND: normal diet; WD+Ch: WD with cholestyramine; GTT: glucose tolerance test.