fig2
Figure 2. AT2R-mediated vasodilation declines with age in female mice only. Second- and third-order mesenteric resistance arteries were isolated from 3-, 12-, and 18-month-old C57/Bl6 (A) female and (B) male mice. Vessels were pre-treated with losartan for 30 min to block angiotensin II type 1 receptors. Following preconstriction to phenylephrine (PE), vasorelaxation in response to Compound 21 (AT2R agonist, 1 × 10-12 to 1 × 10-7 M) was determined by wire myography. Data are expressed as percent relaxation from PE preconsriction. N = 3-7/group (18-month-old males and females = 3/group; 3-month-old females and males and 12-month-old males = 4/group; 12-month-old females = 7/group). The main effects of concentration and age and the interaction effect of these two factors were assessed via repeated measures two-way ANOVA with Tukey post hoc testing where appropriate. Data are means ± SEM,
