fig3

Figure 3. Qualitative analysis of fluorescent rEVs across different platforms. Dot plots from the PBS buffer control, here used as dilution buffer (left), and fluorescent rEVs (right) are shown for eGFP fluorescence (measured under FITC-H) vs. light scattering (either SSC-H, FSC-H, or VSSC-H) as indicated for each plot. Measurements (A) on the NF with an SSC threshold; (B) on the IF with an SSC threshold; (C) on the IF with an FL threshold; (D) on the CF with an SSC threshold; and (E) on the CF with an FL threshold. Total number of events measured is indicated in each dot plot. The rEV samples were pre-diluted before measurements; dot plots shown correspond to a 1:1,000 dilution for NF and a 1:32,000 dilution for both IF and CF. The data shown is representative of two independently performed experiments acquiring 6 diluted samples from the same stock on each instrument. NF: NanoFCM; IF: BD Influx; CF: CytoFLEX LX; SSC: side scatter; SSC-H: side scatter height; VSSC-H: violet side scatter height; eGFP: enhanced green fluorescent protein; FL: fluorescence; FSC-H: forward scatter height; PBS: phosphate-buffered saline; rEVs: recombinant extracellular vesicles.