fig4

Figure 4. Influence of pH, salt, and detergent on L. plantarum growth. OD600 measurements of L. plantarum cultures grown in media (A) adjusted to pH values ranging within 4.74-7.35 (pH measured at the end of culture), (B) supplemented with NaCl concentrations of 0.15-1.0 M, and (C) supplemented with SDS concentrations of 0-8.9 mM. Experiment was performed in triplicate (three biological repeats) and three technical repeats. Data were analyzed using two-way ANOVA followed by Dunnett’s multiple-comparisons test (see statistical analysis in the text). L. plantarum: Lactiplantibacillus plantarum; SDS: sodium dodecyl sulfate; ANOVA: analysis of variance; MRS-T: De Man–Rogosa–Sharpe broth supplemented with Tween 80.