fig1

Figure 1. Liver cholestasis leading to functional impairment of the kidney. (A) Histological examination of liver tissue morphology by H&E staining and Sirius Red staining (scale bar = 50 μm); (B) Histological examination of kidney tissue morphology by H&E staining (top image scale bar = 50 μm, bottom image scale bar =100 μm); (C) Serum ALT level in different groups (n = 6); (D) Serum AST level in different groups (n = 6); (E) Serum Cr level in different groups (n = 6); (F) Serum BUN level in different groups (n = 6). Data were analyzed by an unpaired two-tailed Student’s t-test. Data are presented as mean ± SD. ^*P < 0.05; ^***P < 0.001. H&E: Hematoxylin and eosin; ALT: alanine aminotransferase; AST: aspartate transaminase; Cr: creatinine; BUN: blood urea nitrogen; BDL: bile duct ligation.