fig9

Figure 9. The function of EPCAM⁺ sEV. (A) The western blotting analysis of EPCAM in 293T cells stably transfected with shNC or shEPCAM. The sEVs were isolated from 293T cells stably transfected with shNC or shEPCAM, namely shNC-sEV or shEPCAM-sEV, respectively. TPC-1 and BCPAP cells were treated with shNC-sEV or shEPCAM-sEV; (B and C) TC cell proliferation determined by CCK-8 assays; (D and E) TC cell proliferation determined by colony formation assays; Representative images of migration assays (F and G) and invasion assays (H and I) of TC cells subjected to shNC-sEV or shEPCAM-sEV. The data was analyzed using the Student’s t-test. ^*P value < 0.05; ^**P value < 0.01; ^***P value < 0.001; ns: not significant. EPCAM: Epithelial cell adhesion molecule; sEVs: small extracellular vesicles; shNC: lentivirus of negative control short hairpin RNA; shEPCAM: lentivirus of EPCAM short hairpin RNA; TC: thyroid carcinoma; CCK-8: cell counting kit-8; OD450: optical density at 450 nm.