fig7

Figure 7. The function of HC-derived plasma sEVs in vitro. (A) Dio-labeled plasma sEVs from HC were taken up by TC cells whose nuclei were stained using DAPI; (B) CCK-8 analysis of TC cells treated with PBS or HC-derived plasma sEVs; Representative images of colony formation assays (C and D), migration assays (E and F), and invasion assays (G and H) of TC cells exposed to PBS or HC-derived plasma sEVs. The data was analyzed using the Student’s t-test. ^*P value < 0.05; ^**P value < 0.01; ^***P value < 0.001; ns: not significant. HC: Healthy controls; sEVs: small extracellular vesicles; TC: thyroid carcinoma; DAPI: 4′,6-diamidino-2-phenylindole; CCK-8: cell counting kit-8; PBS: phosphate-buffered saline; DIO: 3,3′-dioctadecyloxacarbocyanine perchlorate; OD450: optical density at 450 nm.