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Figure 3. Survival-Linked EV Signatures. (A) ELISA quantification and Kaplan-Meier analysis of RAB14, FAM3C, and SCAMP3 expression. Statistical significance is indicated as ^**P < 0.01; and ^***P < 0.001, calculated using two-sided Wilcoxon rank-sum tests; (B) Comparison of EV protein detection across three independent ovarian cancer EV datasets; (C) GEPIA2 mRNA expression TCGA analysis of selected markers (CORO1B, LAMP2, MSLN) in tumor samples vs. matched healthy samples and GTEx data (boxes = interquartile range; line = median). Statistical significance: ^*P < 0.05, calculated by one-way ANOVA on log2(TPM + 1)-transformed data. The corresponding GEPIA2 Kaplan-Meier plot with a 75% high-expression cut-off is shown (log-rank test; details see Supplementary Figure 2). EV: Extracellular vesicle; ELISA: enzyme-linked immunosorbent assay; GEPIA2: Gene Expression Profiling Interactive Analysis 2; TCGA: The Cancer Genome Atlas; GTEx: Genotype-Tissue Expression; IQR: interquartile range; ANOVA: analysis of variance; TPM: transcripts per million.