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Figure 4. ALDH2 regulates MTS expression of DNMT1 in cardiomyocytes. (A) Genotype-Tissue Expression (GTEx) database establishes the expression correlation between ALDH2 and DNMT1. (B) Western blot analysis of the 180 and 130-kDa isoforms of DNMT1 subcellular location in WT and KO cardiomyocytes. (C) Immunofluorescent staining of DNMT1 and mitochondria in cardiomyocytes. Scale bar 50 μm. (D) Statistical analysis of MTS expression of DNMT1 in WT and KO cardiomyocytes. (E) Dot blot analysis of 5mc in isolated mtDNA of WT and KO mitochondria. (F) TFAM expression analysis in isolated WT and KO mitochondria. All results are presented as mean ± SEM. Statistical significance for comparisons between two groups was determined by unpaired two-tailed Student’s t-test. **P < 0.01.