REFERENCES
1. Alexy T, Detterich J, Connes P, et al. Physical properties of blood and their relationship to clinical conditions. Front Physiol. 2022;13:906768.
2. Théry C, Zitvogel L, Amigorena S. Exosomes: composition, biogenesis and function. Nat Rev Immunol. 2002;2:569-79.
3. Boulanger CM, Raposo G, Théry C. Extracellular vesicles: How to pass in a few decades from a status of cellular debris, or worse, garbage bags, to that of inter-cellular messengers. Med Sci. 2021;37:1089-91.
4. Lo Cicero A, Stahl PD, Raposo G. Extracellular vesicles shuffling intercellular messages: for good or for bad. Curr Opin Cell Biol. 2015;35:69-77.
5. Stevens KG, Pukala TL. Conjugating immunoassays to mass spectrometry: solutions to contemporary challenges in clinical diagnostics. Trends Analyt Chem. 2020;132:116064.
6. Mann M, Jensen ON. Proteomic analysis of post-translational modifications. Nat Biotechnol. 2003;21:255-61.
7. Pinto AFM, Diedrich JK, Moresco JJ, Yates JR 3rd. Differential precipitation of proteins: a simple protein fractionation strategy to gain biological insights with proteomics. J Am Soc Mass Spectrom. 2023;34:2025-33.
8. Matthiae M, Zhu X, Marie R, Kristensen A. In-line whole blood fractionation for Raman analysis of blood plasma. Analyst. 2019;144:602-10.
9. Hsieh SY, Chen RK, Pan YH, Lee HL. Systematical evaluation of the effects of sample collection procedures on low‐molecular‐weight serum/plasma proteome profiling. Proteomics. 2006;6:3189-98.
10. Molloy MP, Hill C, O’Rourke MB, et al. Proteomic analysis of whole blood using volumetric absorptive microsampling for precision medicine biomarker studies. J Proteome Res. 2022;21:1196-203.
11. Lee LM, Bhatt KH, Haithcock DW, Prabhakarpandian B. Blood component separation in straight microfluidic channels. Biomicrofluidics. 2023;17:054106.
12. Hong K, Iacovetti G, Rahimian A, et al. Clinical evaluation of the Torq Zero Delay Centrifuge System for decentralized blood collection and stabilization. Diagnostics. 2021;11:1019.
13. Gallart-Palau X, Serra A, Wong AS, et al. Extracellular vesicles are rapidly purified from human plasma by PRotein Organic Solvent PRecipitation (PROSPR). Sci Rep. 2015;5:14664.
14. Gallart-Palau X, Serra A, Sze SK. Enrichment of extracellular vesicles from tissues of the central nervous system by PROSPR. Mol Neurodegener. 2016;11:41.
15. Mulet M, Sánchez Milán JA, Lorca C, et al. Oral microbiome-derived proteins in brain extracellular vesicles circulate and tie to specific dysbiotic and neuropathological profiles in age-related dementias. Mol Cell Proteomics. 2025;24:101464.
16. Morales-Sanfrutos J, Etxeberria-Ugartemendia J, Barroso-Gomila O, et al. Defining the reference proteomes for small extracellular vesicles and non-vesicular components. Nat Cell Biol. 2026;28:622-39.
17. Théry C, Witwer KW, Aikawa E, et al. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles. 2018;7:1535750.
18. Clayton A, Boilard E, Buzas EI, et al. Considerations towards a roadmap for collection, handling and storage of blood extracellular vesicles. J Extracell Vesicles. 2019;8:1647027.
19. Lucien F, Gustafson D, Lenassi M, et al. MIBlood‐EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research. J Extracell Vesicles. 2023;12:e12385.
20. Arraud N, Linares R, Tan S, et al. Extracellular vesicles from blood plasma: determination of their morphology, size, phenotype and concentration. J Thromb Haemost. 2014;12:614-27.
21. Maas SL, Broekman ML, de Vrij J. Tunable resistive pulse sensing for the characterization of extracellular vesicles. Methods Mol Biol. 2017;1545:21-33.
22. Gilbile D, Docto D, Kingi DT, et al. How well can you tailor the charge of lipid vesicles? Langmuir. 2019;35:15960-9.
23. Buzás EI, Tóth EÁ, Sódar BW, Szabó-Taylor KÉ. Molecular interactions at the surface of extracellular vesicles. Semin Immunopathol. 2018;40:453-64.
24. Li H, Chen Y, Deng Y, Wang Y, Ke X, Ci T. Effects of surface charge of low molecular weight heparin-modified cationic liposomes on drug efficacy and toxicity. Drug Dev Ind Pharm. 2017;43:1163-72.
25. Kamat NP, Tobé S, Hill IT, Szostak JW. Electrostatic localization of RNA to protocell membranes by cationic hydrophobic peptides. Angew Chem Int Ed Engl. 2015;54:11735-9.
26. Shaba E, Vantaggiato L, Governini L, et al. Multi-Omics integrative approach of extracellular vesicles: a future challenging milestone. Proteomes. 2022;10:12.
27. Skotland T, Sagini K, Sandvig K, Llorente A. An emerging focus on lipids in extracellular vesicles. Adv Drug Deliv Rev. 2020;159:308-21.
28. Pienimaeki-Roemer A, Kuhlmann K, Böttcher A, et al. Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets. Transfusion. 2015;55:507-21.
29. Falabella M, Vernon HJ, Hanna MG, Claypool SM, Pitceathly RDS. Cardiolipin, mitochondria, and neurological disease. Trends Endocrinol Metab. 2021;32:224-37.
30. Chico Y, Abad-García B, Ochoa B, Martínez MJ. Lipidomic data uncover extensive heterogeneity in phosphatidylcholine structural variants in HepG2 cells. Data Brief. 2019;27:104608.
31. Lorca C, Serra A, Gallart-Palau X. Mapping the oxidative stress metabolome in neurology by gas chromatography-mass spectrometry: a systematic review on signature-driven diagnosis and disease monitoring. Redox Biol. 2025;88:103925.
32. Anand S, Samuel M, Kumar S, Mathivanan S. Ticket to a bubble ride: Cargo sorting into exosomes and extracellular vesicles. Biochim Biophys Acta Proteins and Proteom. 2019;1867:140203.
33. Tschirhart BJ, Lu X, Gomes J, et al. Annexin A5 inhibits endothelial inflammation induced by lipopolysaccharide-activated platelets and microvesicles via phosphatidylserine binding. Pharmaceuticals. 2023;16:837.
