fig4

Figure 4. GLI-specific decoy ODN treatment induced apoptosis in the Huh-7 cell line. (A) The dot plots for flow cytometry analysis of apoptotic cells stained by annexin V/PI; (B) The apoptosis rate significantly increased in GLI-specific decoy ODN-treated cells compared to the control and vehicle groups (Early apoptosis: Control: 4.37%, Vehicle: 5.51%, GLI-decoy: 6.09%, Late apoptosis: Control: 10.09%, Vehicle: 8.85%, GLI-decoy: 25.9%). Data are presented as the mean ± SD for three independent experiments. *P ≤ 0.05, **P ≤ 0.01. GLI: Glioma-associated oncogene; ODN: oligodeoxynucleotides; SD: standard deviation.